Changhui Deng

Changhui Deng

1700 4th Street, Box 2542
San Francisco, CA 94143-2542
Cell: (415) 203-1131
Email: chhdeng@gmail.com

EDUCATION

• Ph.D.in Biochemistry & Molecular Biology, 2006, University of California - Riverside, California, USA

• M.S. in Organic Chemistry, 1998, Tsinghua University, Beijing, China

• B.S. in Applied Chemistry, 1995, Nankai University, Tianjin, China

WORK EXPERIENCE

• Apr 2010 to Present, Professional Research Series

Department of Biochemistry & Biophysics, University of California, San Francisco, CA

Develop high-throughput methods for screening aging-related genes which influence life span and anti-aging drugs which can promote longevity, and study their cellular and molecular mechanisms.

• Apr 2006 to Apr 2010, Postdoctoral Scholar

Laboratory of Biological Mass Spectrometry, Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143, USA

Develop a “See & Catch” method, which combines mass spectrometry and fluorescence microscopy techniques, to tag and purify proteins of interest, and explore proteins and their complexes under both static and dynamic conditions.

• Sep 2000 to Mar 2006, PhD Student, Research Assistant & Teaching Assistant

Department of Biochemistry, University of California, Riverside, CA 92521, USA

Study the effects of prolactin on gene expression, the signaling pathways and molecular mechanisms in bone cells.

• Jun 1998 to Aug 2000, Engineer

Beijing Tsinghua Yetop Liquid Crystal Materials Co., Ltd., Beijing 10084, P.R.China

Develop methods for synthesizing the intermediates of liquid crystal materials, design and set up industrial reaction equipment, train technicians and workers

• Sep 1995 to Jun 1998, M.S. Student

Department of Chemistry, Tsinghua University, Beijing 10084, P.R.China

Develop methods for multiple-step whole synthesis of phosphites and N-phosphoamino acids; purify them by distillation, recrystalization, HPLC and column chromatography; characterize them by ¹H-, ¹³C- and ³¹P-NMR, MS, UV and IR

• Sep 1991 to Jun 1995, B.S. student

Department of Chemistry, Nankai University, Tianjin 300071, P.R.China

Develop novel carrier-supported copper catalysts for selective hydrogenation of maleic anhydride to produce tetrahydrofuran or γ-butyrolactone, analyze their specificity by gas chromatography (GC)

SUMMARY OF SKILLS

Molecular Biology & Biochemistry

Cells:

• Culture Primary Cells, Mammalian Cell Lines, Yeast Cell, E.Coli Cell

• Transient Transfection, Stable Transfection for Generating Stable Cell Lines, RNA interference (RNAi -- siRNA & shRNA), Gene Tagging, Gene Knockout, Competent Cell Preparation and Transformation (Yeast & E. coli)

Proteins:

• Extraction of Cytoplasmic, Nuclear, Mitochondrial, Endoplasmic Reticulum, Plasma Membrane and Total Protein

• Immuno-precipitation (IP) & Co-IP Purification of Protein Complexes

• Native and SDS-PAGE Electrophoresis, EMSA, Western Blotting, Silver Staining, Coomassie Brilliant Blue Staining

• ELISA, Luciferase Reporter Assay

RNA & DNA:

• Extraction and Purification of Total RNA, mRNA, Genomic DNA and Plasmid DNA

• Library Preparation for Next-Generation Sequencing (DNA-Seq, RNA-Seq, Ribosome Profiling)

• Electrophoresis, Northern and Southern Blotting

• Primer Design, Reverse Transcription, Real-Time Q-PCR

• DNA Cloning, Plasmid Construction

• Gel Shift (EMSA) /Gel Super Shift Assays

Imaging:

• Immunostaining, Nuclear Staining, Mitochondria Staining, Lysosome Staining

• Cell Flow Cytometry, Fluorescence-Activated Cell Sorting (FACS)

• Microscopy (Zeiss & Nikon Microscopes: Bright Field, Laser Scanning and Spinning Disk Confocal)

• Microfluidics

Mass Spectrometry & Proteomics

Identifying Protein (Complexes), Mapping Posttranslational Modification (Phosphorylation, Methylation, Acethylation, Ubiquitination and SUMOylation):

• Have hands-on experience of operating and maintaining the following mass spectrometers:

• MALDI-TOF MS (Perkin Elmer prOTOF)

• MALDI Ion-Trap MS/MS and MSⁿ (Thermo Finnigan vMALDI-LTQ)

• nano-ESI LC/MS/MS (ABI/MDS SCIEX 4000 Q TRAP LC/MS/MS)

• Be familiar with the following protein identification engines and proteomic tools:

     X! Tandem, Matrix Science-Mascot, Prowl (Chait Lab: ProFound ProteinInfo PeptideMap PepFrag), and ProteinProspector (UCSF)

Chemistry

• HPLC, GC and Column Chromatography

• MS, NMR, IR and UV Spectra

PEER REVIEWED PUBLICATIONS

1. Ke Zou, Silvia Rouskin, Kevin Dervishi, Mark A McCormick, Arjun Sasikumar, Changhui Deng, Zhibing Chen, Matt Kaeberlein, Rachel B Brem, Michael Polymenis, Brian K Kennedy, Jonathan S Weissman, Jiashun Zheng, Qi Ouyang, Hao Li, Life span extension by glucose restriction is abrogated by methionine supplementation: Cross-talk between glucose and methionine and implication of methionine as a key regulator of life span, Science Advances, 6(32):eaba1306, 2020

2. Changhui Deng, Andrew N. Krutchinsky, See & Catch Method for Studying Protein Complexes in Yeast Cells: A Technique Unifying Fluorescence Microscopy and Mass Spectrometry, Methods in Molecular Biology, 1163:75-95, 2014 (Chapter 6 of Yeast Protocols by Wei Xiao, 3^rd Edition, Humana Press)

3. Dunyong Tan, KuanHui E. Chen, Changhui Deng, Peizhi Tang, Jianjun Huang, Trina Mansour, Richard A. Luben, Ameae M. Walker, An N-terminal splice variant of human Stat5a that interacts with different transcription factors is the dominant form expressed in invasive ductal carcinoma, Cancer Letters, 346(1):148-57, 2014

4. Arabinda Nayak, Bassam Berry, Michel Tassetto, Mark Kunitomi, Ashley Acevedo, Changhui Deng, Andrew Krutchinsky, John Gross, Christophe Antoniewski and Raul Andino, Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila, Nature Structural & Molecular Biology, 17(5):547-54, 2010

5. Gang Li, Nga Bien-Ly, Yaisa Andrews-Zwilling, Qin Xu, Aubrey Bernardo, Karen Ring, Brian Halabisky, Changhui Deng, Robert W. Mahley, and Yadong Huang, GABAergic Interneuron Dysfunction Impairs Hippocampal Neurogenesis in Adult Apolipoprotein E4 Knock-in Mice, Cell Stem Cell, 5(6):634-645, 2009

6. Changhui Deng, Xinghua Xiong and Andrew N. Krutchinsky, Unifying Fluorescence Microscopy and Mass Spectrometry for Studying Protein Complexes in Cells, Molecular & Cellular Proteomics, 8(6):1413–1423,2009

7. Changhui Deng, Eric Ueda, KuanHui E. Chen, Craig M. Bula, Anthony W. Norman, Richard A. Luben, Ameae M. Walker, Prolactin Blocks Nuclear Translocation of VDR by Regulating its Interaction with BRCA1 in Ros 17/2.8 Osteosarcoma Cells, Molecular Endocrinology, 23(2):226–236, 2009 (Highlighted in the monthly Endocrine News, February 2009, P24)

8. Yongqiang Wang, Mingxiang Liao, Nicholas Hoe, Poulomi Acharya, Changhui Deng, Andrew N. Krutchinsky and Maria Almira Correia, A Role for Protein Phosphorylation in Cytochrome P450 3A4 Ubiquitin-Dependent Proteasomal Degradation, Journal of Biological Chemistry, 284(9):5671-84, 2009

9. Justin D. Blethrow, Chao Tang, Changhui Deng, Andrew N. Krutchinsky, Modular Mass Spectrometric Tool for Analysis of Composition and Phosphorylation of Protein Complexes, PLoS ONE, 2007 Apr 4;2:e358

10. Xiaolei Xu, Wei Wu, Valencia Williams, Amy Khong, Yen-Hao Chen, Changhui Deng, Ameae M. Walker, Opposite Effects of Unmodified Prolactin and a Molecular Mimic of Phosphorylated Prolactin on Morphology and the Expression of Prostate Specific Genes in the Normal Rat Prostate, Prostate, 54(1):25-33, 2003

11. Changhui Deng, Yanmei Li, Yufen Zhao, Formation of Oligopeptides from N-Phosphoamino Acid by Ifrared Radiation, Phosphorus, Sulfur and Silicon and the Related Elements, 163:203-210, 2000

12. Ning Xu, Zhou Zhou, Hongmei Wang, Changhui Deng, Yanmei Li, Yufeng Zhao, Lingxuan Wang , Tiankai Li, Chiral Discrimination in N-(O,O-dialkyl) Phosphoamino Acid Monolayers at the Air–Water Interface, Chinese Chemical Letter, 11 (8):717-718, 2000

13. Changhui Deng, Yanmei Li, Ning Xu and Yufen Zhao, Synthesis of N-Phosphoamino Acids with Long Dialkoxyl Chains, Journal of Chemical Research (S):589, 1999; Journal of Chemical Research (M): 2552-2560, 1999

14. Shuji Zhang*, Weijun Zhao*, Changhui Deng**, Meng Zhao, etc, Study on Carrier-supported Copper Catalysts for Selective Hydrogenation of Maleic Anhydride to Produce Tetrahydrofuran and γ-Butyrolactone, Speciality Petrochemicals, No. 1:10-13, 1996 (* Supervisors, ** major contributor of this work)